Mouse anti MyoD1

Here you can find detailed information about selected products. Contact with us if you have nay questions to product or order proces.

Main information

NameMouse anti MyoD1
Catalog numberX1414M
Size100 μg
Price€ 426.00


More details

CategoryPrimary Antibodies
Long descriptionRecognizes a protein of 45kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of WilmÕs tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, EwingÕs sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of WilmÕs tumors.
Antibody come fromHybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 523-768 of human CAP-E and mouse myeloma cells.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Clone5.2F
Antigen-antibody binding interactionMouse anti MyoD1 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman, Mouse, Rat, Chicken
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationELISA, Western Blot, Immunoprecipitation, Immunohistochemistry (Frozen and Paraffn Sections), Immunofluorescense
Antibody's suited forThis antibody can be used for Western blotting (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Storage-20ºC
Relevant references1. Hirano, T.R., et al. 'Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosphila Barren protein.' Cell, 1997, 89, 511-521._x000B__x000B_2. Schmiesing, J.A., et al. 'A human condensin complex containing hCAP-C, hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation.' Mol. Cell Biol. 2000, 20, 6996-7006
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus