Mouse anti Syk Signaling Protein

Here you can find detailed information about selected products. Contact with us if you have nay questions to product or order proces.


Main information

NameMouse anti Syk Signaling Protein
Catalog numberX1460M
Size100 μg
Price€ 500.00


More details

CategoryPrimary Antibodies
Long descriptionRecognizes a protein of 72kDa which is identified as Syk, a protein tyrosine kinase (PTK). It reacts with both the un(der)phosphorylated as well as the phosphorylated form of Syk protein. MAb 4D10.1 is highly specific to p72Syk and shows no cross-reaction with ZAP-70 or other related protein tyrosine kinases. Syk protein is expressed in immature T cells, all B cells, basophils, hepatocytes, platelets, and erythrocytes. It functions in some T cell receptor signalling processes in addition to B cell receptor and integrin-mediated signal transduction processes. The 4D10.1 MAb supports in vitro kinase activity.
Antibody come fromHybridoma produced by the fusion of splenocytes from immunized with full length wild-type human p53 protein and mouse myeloma cells.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Clone4D10.1
Antigen-antibody binding interactionMouse anti Syk Signaling Protein Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot, Immunoprecipitation, Immunohistochemistry, Enzyme Immunoassay_x000B__x000B_
Antibody's suited forThis antibody can be used for Western blotting (0.5-1 µg/ml), immunohistochemistry on formalin/paraffain embedded tissues (1-2 µg/ml) and immunoprecipitation (2 µg/mg of protein lysate). Antibody reacts with mutant as well as wild-type p53 protein. Optimal concentration should be evaluated by serial dilutions.
Storage-20ºC
Relevant references1. Zeng, X., et al. UV but not gamma irradiation accelerates p53-induced apoptosis of teratocarcinoma cells by repressing MDM2 transcription. Cancer Res. 2000, 60, 6184-6188_x000B__x000B_2. Hussain, S.P., et al. p53 tumor suppressor gene: at the crossroads of molecular carcinogenesis, molecular epidemiology and human risk assessment. Ann. N.Y. Acad. Sci. 2000, 919, 79-85_x000B__x000B_3. Hellin, A.C., et al. Roles of Nuclear Factor-kappaB, p53 and p21/WAF1 in Daunomycin-induced cell cycle arrest and apoptosis. J. Pharmacol. Exp. Ther. 2000, 295, 870-878_x000B__x000B_4. Barkte, J., et al. 'Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies.'
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Cell nucleus signaling proteins and molecules are part of a complex system of communication that governs basic cellular activities and coordinates cell actions. The ability of cells to perceive and correctly respond to their microenvironment is the basis of development, tissue repair, and immunity as well as normal tissue homeostasis. Errors in cellular information processing are responsible for diseases such as cancer, autoimmunity, and diabetes. By understanding cell signaling, diseases may be treated effectively and, theoretically, artificial tissues may be created.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus