Mouse anti V-CAM-1 (CD106)

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Main information

NameMouse anti V-CAM-1 (CD106)
Catalog numberX1473M
Size100 μg
Price€ 500.00

More details

CategoryPrimary Antibodies
Long descriptionIt recognizes an integral membrane glycoprotein of 110kDa, identified as vascular cell adhesion molecule (VCAM-1). VCAM-1 is distributed across non-leukocyte and leukocyte cells. Its expression is reportedly correlated with adhesion of melanoma, BurkittÕs lymphoma, osteosarcoma, and kidney carcinoma but not colon carcinoma cells to endothelial cells which may determine the site of tumor metastasis.
Antibody come fromn/a
Other descriptionProvided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.
Antigen-antibody binding interactionMouse anti V-CAM-1 (CD106) Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry
Antibody's suited forThis CD15 antibody is an IgG3 antibody as compared to other CD15 antibodies available which are IgMÕs. These IgMÕs cause the cells labeled with them to aggregate in the cytometer and read as one cell, producing lower numbers of cells to be reported. Our CD15 antibody does not cause the cells to aggregate, yielding much more accurate results.
Relevant references1. Ball ED. M5. CD15 cluster workshop report. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, et al. editors. Leucocyte typing V. White cell differentiation antigens. Proceedings of Fifth International Workshop and conference held in Boston, USA 3-7 november, 1993. Volume One. Oxford University Press (1995)._x000B_2. Bahia DMM, Yamamoto M, Chauffaille MLF, Kimura EYS, Bordin JO, et al. Aberrant phenotypes in acute myeloid leukemia: a high frequency and clinical significance. Haematologica.86: 801-806 (2001)_x000B_3. van Gongen JJM, Adriaansen HJ. Immunobiology of leukemia. In Henderson ES, Lister TA, Greaves MF editors. Leukemia. WB Saunders Company (1996)_x000B_4. MenŽndez P, et al. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry (Comm. Clin. Cytometry) 34: 264-271 (1998)_x000B_5. GratamaJW, MenŽndez P, Kraan J, Orfao A. Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erytrocyte lysing reagents. J Immunol. Methods 239: 13-23 (2000)_x000B_6. Protection of Laboratory Workers from occupationally acquired infections. Second edition; approved guideline (2001). Villanova PA: National Committee for Clinical Laboratory Standards; Document M29-A2._x000B_7. Procedures for the collection of diagnostic blood specimens by venipuncture- approved standard; Fifth edition (2003). Wayne PA: National Committee for Clinical Laboratory Standards; Document H3-A5._x000B_8. Clinical applications of flow cytometry: Quality assurance and immunophenotyping of lymphocytes; approved guideline (1998). Wayne PA: National Committee for Clinical Laboratory Standards; Document H42-A._x000B_9. Braylan RC, Orfao A, Borowitz MJ, Davis BH. Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting. Cytometry 46: 23-7 (2001)_x000B_10. Jennings CD, Foon KA. Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy. Blood 90(8): 2863-2892 (1997)_x000B_11. Orfao A, Ortu–o F, de Santiago M, L—pez A, San Miguel J. Immunophenotyping of acute leucemias and myelodysplastic syndromes. Cytometry Part A 58A: 62-71 (2004)_x000B_12. Reichert et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol 60:190-208 (1991)
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus