Mouse anti c-HER2/neu

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Main information

NameMouse anti c-HER2/neu
Catalog numberX1566M
Size100 μg
Price€ 500.00


More details

CategoryPrimary Antibodies
Long descriptionc-erbB-2 is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein. This antibody reacts with the intact cells,1,2 slightly increases (~2-fold) the tyrosine kinase activity,1 accelerates (~50%) c-erbB-2 turnover,1 and inhibits (~80%) tumorigenic growth in CD1/nude mice of N87 human gastric carcinoma cell line overexpressing c-erbB-2.1
Antibody come fromSynthetic peptide derived from the intermediate region of the EDG6 (S1P4) protein.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
CloneN24
Antigen-antibody binding interactionMouse anti c-HER2/neu Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationImmunofluorescense, Immunoprecipitation
Antibody's suited forAntibody can be used for Western blotting (5-10 µg/ml). Detects an approximately 42 kDa band on RH7777 cells transfected with full-length EDG-6. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.
Storage-20ºC
Relevant references1. Yamazaki, Y., et al. 'EDG-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway.' Biochem. Biophys. Res. Commun. 2000, 268, 583-589._x000B__x000B_2. Van Brocklyn, J.R., et al. 'Sphingosine 1-phosphate is a ligand for the G protein-coupled receptor EDG-6.' Blood 2000, 95, 2624-2629._x000B__x000B_3. Takuwa, Y., et al. 'Subtype-specific, differential activities of the EDG family receptors for sphingosine 1-phosphate, a novel lysophospholipid mediator.' Mol. Cell Endocrinol. 2001, 177, 3-11.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus