Mouse anti GFP (Green Fluorescent Protein)

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Main information

NameMouse anti GFP (Green Fluorescent Protein)
Catalog numberX1574M
Size100 μg
Price€ 304.00


More details

CategoryPrimary Antibodies
Long descriptionGreen Fluorescent Protein (GFP) from Jellyfish (Aequorea Victoria) is a 27kDa protein that provides strong visible fluorescence. GFP can be used as a reporter of gene expression, a tracer of cell lineage, and a fusion tag to monitor protein localization from living cells. It can also be used to access protein-protein interactions in yeast two hybrid systems and measure distances between proteins in fluorescence energy transfer (FRET).
Antibody come fromHybridoma produced by the fusion of splenocytes from BALB/c mice immunized with rat brain synaptosomal membranes and mouse NS1 cells.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
CloneF56-BA1.2.3
Antigen-antibody binding interactionMouse anti GFP (Green Fluorescent Protein) Antibody
Antibody is raised inMouse
Antibody's reacts withN/A
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot
Antibody's suited forAntibody can be used for Western blotting (1-5 µg/ml) and immunohistochemistry on paraformaldehyde fixed CNS tissues (1-5 µg/ml). Does not stain reduced proteins. Optimal concentration should be evaluated by serial dilutions.
Storage-20ºC
Relevant references1. Ritchie, T.C., et al. 'A nerve terminal protein with a selective distribution in spinal cord and brain.' J. Neurosci. 1989: 9, 2697-2709_x000B__x000B_2. Zhao, N., et al. 'Cloning and sequence analysis of the human SNAP25 cDNA.' Gene 1994: 145, 313-314_x000B__x000B_3. McMahon , H.T., et al. 'Synaptic core complex of synaptobrevin, syntaxin and SNAP25 forms high affinity alpha-SNAP binding site.' J. Biol. Chem. 1995: 270, 2213-2217_x000B__x000B_4. Gerona, R.R., et al. 'The C terminus of SNAP25 is essential for Ca(2+)-dependent binding of synaptotagmin to SNARE complexes.' J. Biol. Chem. 2000: 275, 6328-6336
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus