AKT1 Mouse monoclonal antibody

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Main information

NameAKT1 Mouse monoclonal antibody
Catalog numberMB9025-50ul
Price€ 408.00

More details

Alternative NameRAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα;
ImmunogenThis antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of AKT1.
ReactivityHuman, mouse
ApplicationsWB ICC IHC FC
Application_allWB: 1:2000 IHC/ICC: 1:100-1:200 FC: 100-1:200
ProductMouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&PurityProtein A affinity purified
Storage&StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
SpecificityThis antibody detects endogenous levels of AKT1 and does not cross-react with related proteins.
UsageWestern blot (WB) analysis of AKT1 Mouse mAb at 1:2000 dilution Lane1:HepG2 whole cell lysate Lane2:MCF7 whole cell lysate Lane3:Hela whole cell lysate
BiowMW~ 60 kDa
NoteFor research use only, not for use in diagnostic procedure.
PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus
French translationanticorps