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| Name | AKT2 (phospho-S474) polyclonal antibody |
|---|---|
| Catalog number | BS4720-100ul |
| Size | 100ul |
| Price | € 448.00 |
| Alternative Name | RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; |
|---|---|
| Immunogen | Synthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474. |
| Swiss-Prot | P31751 |
| Reactivity | Human,Mouse,Rat |
| Applications | WB IHC |
| Application_all | WB: 1:500~1:1000 IHC: 1:50~1:200 |
| Product | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Purification&Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is> 95% (by SDS-PAGE). |
| Storage&Stability | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Specificity | p-AKT2 (S474) polyclonal antibody detects endogenous levels of AKT2 protein only when phosphorylated at Ser474.This antibody does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues. |
| Usage | Western blot (WB) analysis of p-AKT2(S474) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,15 minutes) whole cell lysate(40ug) Lane3:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug) Lane4:The Brain tissue lysate of Rat(40ug) Lane5:The Brain tissue lysate of Mouse(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:AML-12 whole cell lysate(40ug) |
| BiowMW | ~ 60 kDa |
| Note | For research use only, not for use in diagnostic procedure. |
| Properties | If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. |
| Group | Polyclonals and antibodies |
| About | Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. |
| French translation | anticorps |