AKT2 (phospho-S474) polyclonal antibody

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Main information

NameAKT2 (phospho-S474) polyclonal antibody
Catalog numberBS4720-100ul
Price€ 448.00

More details

Alternative NameRAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ;
ImmunogenSynthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474.
ApplicationsWB IHC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200
ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is> 95% (by SDS-PAGE).
Storage&StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificityp-AKT2 (S474) polyclonal antibody detects endogenous levels of AKT2 protein only when phosphorylated at Ser474.This antibody does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues.
UsageWestern blot (WB) analysis of p-AKT2(S474) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,15 minutes) whole cell lysate(40ug) Lane3:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug) Lane4:The Brain tissue lysate of Rat(40ug) Lane5:The Brain tissue lysate of Mouse(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:AML-12 whole cell lysate(40ug)
BiowMW~ 60 kDa
NoteFor research use only, not for use in diagnostic procedure.
PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translationanticorps