anti-AKT1 Antibody

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Main information

Nameanti-AKT1 Antibody
Catalog number31-052
Size50 ug
Price€ 180.00


More details

Gene infoAKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Gene namesAKT1, AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
Antibodie's labelunlabelled serum
PurityAntibody is purified by peptide affinity chromatography method.
Ab raised inRabbit
TypePolyclonal
AntigenAntibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKT1.
Immuno applicationELISA, Western Blot
Antibody adviceAKT1 antibody can be used for detection of AKT1 by ELISA at 1:312500. AKT1 antibody can be used for detection of AKT1 by western blot at 0.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Suspention, pH, azideAntibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Keep atFor short periods of storage (days) store at 4 grades C. For longer periods of storage, store AKT1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
Works withHuman, Mouse, Dog
Omim nr62241011
Ncbi nrNP_005154
KDa56 kDa
Vial with antibodyLyophilized
Quantity per ml1 mg/ml
NCBI GENE nr207
GENE symbolAKT1
Antigene namev-akt murine thymoma viral oncogene homolog 1
SourceHomo sapiens
Protein nrP31749
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translationanticorps